Search results for "Degenerate primer"

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easyPAC: A Tool for Fast Prediction, Testing and Reference Mapping of Degenerate PCR Primers from Alignments or Consensus Sequences

2012

Video abstract A video abstract by the authors of this paper is available. video-abstract8870.mov

homologous genesparalogous genesComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISIONlcsh:EvolutionGenomicsComputational biologyBiologyBioinformaticslaw.inventionlawDegenerate primerGeneticsConsensus sequenceSoftware Reviewlcsh:QH359-425De novo sequencingPCR primer predictionEcology Evolution Behavior and SystematicsPolymerase chain reactionSequence (medicine)Degenerate energy levelsalignmentComputer Science Applicationsconsensus sequencedegenerate primersPrimer (molecular biology)Evolutionary Bioinformatics
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Recombinant GII.P16 genotype challenges RT-PCR-based typing in region A of norovirus genome

2021

Abstract Objectives In latest years GII.4[P16] and GII.2[P16] noroviruses have become predominant in some temporal/geographical settings. In parallel with the emergence of the GII.P16 polymerase type, norovirus surveillance activity in Italy experienced increasing difficulties in generating sequence data on the RNA polymerase genomic region A, using the widely adopted JV12A/JV13B primer set. Two sets of modified primers (Deg1 and Deg2) were tested in order to improve amplification and typing of the polymerase gene. Methods Amplification and typing performance of region A primers was assessed in RT-PCR on 452 GII norovirus positive samples obtained from 2194 stool samples collected in 2016–2…

0301 basic medicineMicrobiology (medical)Settore MED/07 - Microbiologia E Microbiologia ClinicaGenotype030106 microbiologymedicine.disease_cause03 medical and health scienceschemistry.chemical_compoundfluids and secretions0302 clinical medicineRNA polymeraseGenotypemedicineHumans030212 general & internal medicineTypingChildPolymerase GenePhylogenyPolymeraseCaliciviridae InfectionsbiologyReverse Transcriptase Polymerase Chain ReactionNorovirusvirus diseasesVirologyInfectious DiseasesReal-time polymerase chain reactionItalychemistryDegenerate primers GII.P16 Norovirus PolymeraseTypingNorovirusbiology.proteinPrimer (molecular biology)Journal of Infection
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